dc.contributor.author | Nyambega, Benson | |
dc.contributor.author | Helbig, Claudia | |
dc.contributor.author | Masiga, Daniel K | |
dc.contributor.author | Clayton, Christine | |
dc.contributor.author | Levin, Mariano J | |
dc.date.accessioned | 2018-06-04T08:27:42Z | |
dc.date.available | 2018-06-04T08:27:42Z | |
dc.date.issued | 2014-03-20 | |
dc.identifier.citation | Nyambega B, Helbig C, Masiga DK, Clayton C, Levin MJ (2014) Proteins Associated with SF3a60 in T. brucei. PLoS ONE 9(3): e91956. https://doi.org/10.1371/journal.pone.0091956 | en_US |
dc.identifier.uri | https://repository.maseno.ac.ke/handle/123456789/539 | |
dc.description | Trypanosoma brucei relies on Spliced leader trans splicing to generate functional
messenger RNAs. Trans splicing joins the specialized SL exon from the SL RNA to pre-
mRNAs and is mediated by the trans-spliceosome, which is made up of small nuclear
ribonucleoprotein particles and non-snRNP factors. Although the trans spliceosome is
essential for trypanosomatid gene expression, not all spliceosomal protein factors are
known and of these, only a few are completely characterized. In this study, we have
characterized the trypanosome Splicing Factor, SF3a60, the only currently annotated SF3a
component. As expected, epitope-tagged SF3a60 localizes in the trypanosome nucleus.
SF3a60 is essential for cell viability but its depletion seem to have no detectable effect on
trans-splicing. In addition, we used SF3a60 as bait in a Yeast-2-hybrid system screen and … | en_US |
dc.description.abstract | Trypanosoma brucei relies on Spliced leader trans splicing to generate functional messenger RNAs. Trans splicing joins the specialized SL exon from the SL RNA to pre-mRNAs and is mediated by the trans-spliceosome, which is made up of small nuclear ribonucleoprotein particles and non-snRNP factors. Although the trans spliceosome is essential for trypanosomatid gene expression, not all spliceosomal protein factors are known and of these, only a few are completely characterized. In this study, we have characterized the trypanosome Splicing Factor, SF3a60, the only currently annotated SF3a component. As expected, epitope-tagged SF3a60 localizes in the trypanosome nucleus. SF3a60 is essential for cell viability but its depletion seem to have no detectable effect on trans-splicing. In addition, we used SF3a60 as bait in a Yeast-2-hybrid system screen and identified its interacting protein factors. The interactions with SF3a120, SF3a66 and SAP130 were confirmed by tandem affinity purification and mass spectrometry. | en_US |
dc.publisher | Public Library of Science | en_US |
dc.title | Proteins Associated with SF3a60 in T. brucei | en_US |
dc.type | Article | en_US |