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dc.contributor.authorNyambega, Benson
dc.contributor.authorHelbig, Claudia
dc.contributor.authorMasiga, Daniel K
dc.contributor.authorClayton, Christine
dc.contributor.authorLevin, Mariano J
dc.date.accessioned2018-06-04T08:27:42Z
dc.date.available2018-06-04T08:27:42Z
dc.date.issued2014-03-20
dc.identifier.citationNyambega B, Helbig C, Masiga DK, Clayton C, Levin MJ (2014) Proteins Associated with SF3a60 in T. brucei. PLoS ONE 9(3): e91956. https://doi.org/10.1371/journal.pone.0091956en_US
dc.identifier.urihttps://repository.maseno.ac.ke/handle/123456789/539
dc.descriptionTrypanosoma brucei relies on Spliced leader trans splicing to generate functional messenger RNAs. Trans splicing joins the specialized SL exon from the SL RNA to pre- mRNAs and is mediated by the trans-spliceosome, which is made up of small nuclear ribonucleoprotein particles and non-snRNP factors. Although the trans spliceosome is essential for trypanosomatid gene expression, not all spliceosomal protein factors are known and of these, only a few are completely characterized. In this study, we have characterized the trypanosome Splicing Factor, SF3a60, the only currently annotated SF3a component. As expected, epitope-tagged SF3a60 localizes in the trypanosome nucleus. SF3a60 is essential for cell viability but its depletion seem to have no detectable effect on trans-splicing. In addition, we used SF3a60 as bait in a Yeast-2-hybrid system screen and …en_US
dc.description.abstractTrypanosoma brucei relies on Spliced leader trans splicing to generate functional messenger RNAs. Trans splicing joins the specialized SL exon from the SL RNA to pre-mRNAs and is mediated by the trans-spliceosome, which is made up of small nuclear ribonucleoprotein particles and non-snRNP factors. Although the trans spliceosome is essential for trypanosomatid gene expression, not all spliceosomal protein factors are known and of these, only a few are completely characterized. In this study, we have characterized the trypanosome Splicing Factor, SF3a60, the only currently annotated SF3a component. As expected, epitope-tagged SF3a60 localizes in the trypanosome nucleus. SF3a60 is essential for cell viability but its depletion seem to have no detectable effect on trans-splicing. In addition, we used SF3a60 as bait in a Yeast-2-hybrid system screen and identified its interacting protein factors. The interactions with SF3a120, SF3a66 and SAP130 were confirmed by tandem affinity purification and mass spectrometry.en_US
dc.publisherPublic Library of Scienceen_US
dc.titleProteins Associated with SF3a60 in T. bruceien_US
dc.typeArticleen_US


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