Maseno University Repository

Depletion of trypanosome CTR9 leads to gene expression defects

Show simple item record

dc.contributor.author Ouna, Benard A
dc.contributor.author Nyambega, Benson
dc.contributor.author Manful, Theresa
dc.contributor.author Helbig, Claudia
dc.contributor.author Males, Matilda
dc.contributor.author Fadda, Abeer
dc.contributor.author Clayton, Christine
dc.date.accessioned 2018-06-04T07:46:20Z
dc.date.available 2018-06-04T07:46:20Z
dc.date.issued 2012-04-20
dc.identifier.citation Ouna BA, Nyambega B, Manful T, Helbig C, Males M, Fadda A, et al. (2012) Depletion of Trypanosome CTR9 Leads to Gene Expression Defects. PLoS ONE 7(4): e34256. https://doi.org/10.1371/journal.pone.0034256 en_US
dc.identifier.uri https://repository.maseno.ac.ke/handle/123456789/536
dc.description The Paf complex of Opisthokonts and plants contains at least five subunits: Paf1, Cdc73, Rtf1, Ctr9, and Leo1. Mutations in, or loss of Paf complex subunits have been shown to cause defects in histone modification, mRNA polyadenylation, and transcription by RNA polymerase I and RNA polymerase II. We here investigated trypanosome CTR9, which is essential for trypanosome survival. The results of tandem affinity purification suggested that trypanosome CTR9 associates with homologues of Leo1 and Cdc73; genes encoding homologues of Rtf1 and Paf1 were not found. RNAi targeting CTR9 resulted in at least ten- fold decreases in 131 essential mRNAs: they included several that are required for gene expression and its control, such as those encoding subunits of RNA polymerases, exoribonucleases that target mRNA, RNA helicases and RNA-binding proteins … en_US
dc.description.abstract The Paf complex of Opisthokonts and plants contains at least five subunits: Paf1, Cdc73, Rtf1, Ctr9, and Leo1. Mutations in, or loss of Paf complex subunits have been shown to cause defects in histone modification, mRNA polyadenylation, and transcription by RNA polymerase I and RNA polymerase II. We here investigated trypanosome CTR9, which is essential for trypanosome survival. The results of tandem affinity purification suggested that trypanosome CTR9 associates with homologues of Leo1 and Cdc73; genes encoding homologues of Rtf1 and Paf1 were not found. RNAi targeting CTR9 resulted in at least ten-fold decreases in 131 essential mRNAs: they included several that are required for gene expression and its control, such as those encoding subunits of RNA polymerases, exoribonucleases that target mRNA, RNA helicases and RNA-binding proteins. Simultaneously, some genes from regions subject to chromatin silencing were derepressed, possibly as a secondary effect of the loss of two proteins that are required for silencing, ISWI and NLP1. en_US
dc.publisher Public Library of Science en_US
dc.title Depletion of trypanosome CTR9 leads to gene expression defects en_US
dc.type Article en_US


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Maseno University Repository


Browse

My Account