Antimicrobial activity of Eugenia jambolana seeds against foodborne isolates

Abstract

Food borne diseases encompass a wide spectrum of illnesses associated with the ingestion of food contaminated by microbes. Many medicinal plants are considered to be potential antimicrobial crude drugs as well as a source for novel compounds with anti-microbial activity, with possibly new modes of action. Eugenia jambolana has been reported to contain phytochemicals like coumarin, flavanoids, glycosides, phenols, tannins and steroids. Diluted meat samples in PBS were inoculated onto culture plates of S S Agar, and Chromogenic agar and incubated at 370C overnight. After 48 hours incubation, cultures were examined for significant growth. Subcultures were then made into plates of nutrient agar and incubated for another 24 hours. The primary identification of the bacterial isolates was made based on colonial appearance and pigmentation. Antibacterial activities of the plant extracts were tested on Mueller-Hinton agar by well diffusion method. Acetone, methanol and ethanolic Eugenia jambolana extracts against S. aureus, Pseudomonas species and Salmonella species showed a significant zone of inhibition. The objectives of the present study were as follows: Isolation of bacteria from meat, Study of biofilm production by the isolated bacterias and Screening of Eugenia jambolana extract for antibacterial activity against the isolated bacteria.