Gal operon of Streptomyces

Abstract

his invention relates to a recombinant DNA mole cule comprising the Streptomyces gal operon. Hodgson, J. Gen. Micro, 128, 2417-2430 (1982), re port that Streptomyces coelicolor A3 (2) has a glucose repression system which allows repression at the level of transcription of the arabinose uptake system, one of the glycerol uptake systems, and also repression of the galactose uptake system in wild type strains. There is no report in Hodgson of actual galactose metabolism by S. coelicolor A3 (2). Okeda et at. Mol. Gen. Genet, 196, 501-507 (1984), report that glucose kinase activity, 2-deoxyglu-cose sensitivy, glucose utilization and glucose repression were all restored to S. coelicolor A3 (2) glk (glucose kinase) mutants transformed by a 3.5 kb DNA fragment which contained the glk gene cloned from S. coelicolor into a phage vector.