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Prevalence of transmitted drug resistance mutations in HIV-1-infected drug-naive patients from urban and suburban regions of Kenya

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dc.contributor.author Simon Onsongo, Syed Hani Abidi, Samoel Khamadi, Reena Shah, Sheila Kageha, Peter Ojwang, Syed Ali, Nancy Okinda
dc.date.accessioned 2020-11-26T08:09:28Z
dc.date.available 2020-11-26T08:09:28Z
dc.date.issued 2016-03-01
dc.identifier.uri https://repository.maseno.ac.ke/handle/123456789/3023
dc.description.abstract Abstract HIV was first described in Kenya in 1984–1985. Currently, Kenya has an estimated HIV-1 prevalence of 6.2%. With the introduction of antiretroviral drugs, the survival of most HIV patients has been prolonged markedly. However, this is greatly threatened by increasing rates of antiretroviral dug resistance, which may eventually lead to suboptimal treatment outcomes. The objective of this study was to characterize currently occurring antiretroviral drug resistance mutations among drug-naive patients visiting two referral hospitals in Kenya. Using polymerase chain reaction, the HIV protease gene was amplified from blood samples of 63 study participants. The sequences were used to determine HIV-1 subtype and presence/prevalence of mutations associated with resistance to protease inhibitors. Finally, the protease gene was variably measured using Shannon entropy analysis. Analysis of frequency of HIV-1 subtypes revealed subtype A to be the predominant subtype, while the analysis of drug resistance mutations revealed the presence of four minor drug resistance mutations associated weakly with resistance to protease inhibitors. Among these mutations, L33I was the most prevalent mutation. Shannon entropy analysis revealed high genomic variability, especially in region spanning nucleotides 1–55, 113–170, and 205–240. This study warrants the need for dedicated efforts to improve compliance to antiretroviral therapy and reduce transmitted resistance rates, which will greatly ensure the therapeutic efficacy of antiretroviral drugs. en_US
dc.publisher Mary Ann Liebert, Inc. en_US
dc.subject Mutations in HIV-1-Infected Drug-Naive Patients en_US
dc.title Prevalence of transmitted drug resistance mutations in HIV-1-infected drug-naive patients from urban and suburban regions of Kenya en_US
dc.type Article en_US


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