Abstract:
Enzyme linked immunosorbent assay (ELISA) kit is a versatile, cheap and relatively
available tool that can be used in remote areas. In this study, performance of ELISA kit was
evaluated in terms of accuracy, recovery, precision, sensitivity, cross reactivity and matrix
interference for pesticide residue determination in water and sediment samples. This
method was compared with high performance liquid chromatography (HPLC) which is not a
commonly available analytical technique for chlorpyrifos ethyl residue analysis in
developing countries. The ELISA kit had limits of detection (LOD) of 0.37 µg L− 1 and 0.42
µg Kg− 1 dry weight (dw), for chlorpyrifos ethyl in water and sediment samples, respectively
using deionized water and a control sediment sample. Mean percentage recoveries and
coefficients of variation (CV) for ELISA kit varied from 96.0±5.8% to 108.0±3.4% for water …