| dc.description.abstract | Abstract Restriction fragment length polymorphism
(RFLP), ampliWed fragment length polymorphism
(AFLP), expressed-sequenced tag (EST), and simple
sequence repeat (SSR) markers were used to generate
a genetic map of the tetraploid Wnger millet (Eleusine
coracana subsp. coracana) genome (2n = 4x = 36).
Because levels of variation in Wnger millet are low, the
map was generated in an inter-subspeciWc F2 population from a cross between E. coracana subsp. coracana
cv. Okhale-1 and its wild progenitor E. coracana subsp.
africana acc. MD-20. Duplicated loci were used to
identify homoeologous groups. Assignment of linkage
groups to the A and B genome was done by comparing
the hybridization patterns of probes in Okhale-1, MD20, and Eleusine indica acc. MD-36. E. indica is the A
genome donor to E. coracana. The maps span 721 cM
on the A genome and 787 cM on the B genome and
cover all 18 Wnger millet chromosomes, at least partially. To facilitate the use of marker-assisted selection
in Wnger millet, a Wrst set of 82 SSR markers was developed. The SSRs were identiWed in small-insert genomic
libraries generated using methylation-sensitive restriction enzymes. Thirty-one of the SSRs were mapped.
Application of the maps and markers in hybridizationbased breeding programs will expedite the improvement of Wnger millet. | en_US |
