Feasibility of an enhanced washing process to extract PBDEs/heavy metals/antibiotics from antibiotic resistance gene-affected soil with aqueous DNA followed by mi...

Abstract

Abstract Purpose Remediation of polybrominated diphenyl ethers (PBDEs)/heavy metals/antibiotics mixed contaminated soil with high abundance of antibiotic resistance genes (ARGs) has being greatly challenging. Materials and methods An elevated soil washing procedure was developed to investigate the potential of applying DNA solution to extract PBDEs, Cu, Pb, tetracycline, and sulfadiazine from a high abundance of ARG- affected soil, followed by microbial augmentation to restore the microbiological functioning of washed soil. Results and discussion Two successive washings with 15 g L− 1 DNA solution plus ultrasonication (25 kHz for 15 min) were optimal for extracting 80.6% BDE28, 85.2% BDE47, 64.7% Cu, 60.3% Pb, 100% tetracycline, and 100% sulfadiazine. Simultaneously, the ARG abundance decreased to the level of 10− 7–10− 8 (ARGs copies/16S copies) …

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