Sputum Smear Conversion Time of HIV Infected and Uninfected Patients with Rifampicin and Isoniazid Mycobacterium tuberculosis gene Mutations in Western Kenya
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Publication Date
2012Author
Shiluli, Clement
Ouma, Ouma
Vulule, John
Khayumbi, Jeremiah
Murithi, Wilfred
Musau, Susan
Okumu, Albert
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Show full item recordAbstract/ Overview
Mono-resistant and multi-drug resistant tuberculosis (MDR-TB) has
been enhanced by delays in the identification of resistant strains. However, resistance gene
patterns and the extent of monoresistant TB and MDR-TB in western Kenya is unknown. The
objective of the study was to identify cases of mono-resistant TB and MDR-TB in western
Kenya. Methods: Early morning sputum samples were cultured on Mycobacteria growth
indicator tubes (MGIT) and incubated at 37 C. Drug susceptibility testing (DST) using the
SIRE® kit was done on ZN smear positive MGIT tubes and line probe assay (LPA)
performed to identify specific mutations on the rpo B, kat G and inh A genes. Mutations on
discordant samples were confirmed by sequence analysis. Results: The rpo B H526Y and
the kat G S315T1 mutations were common in HIV positive patients (8% and 18 …