Browsing Department of Botany by Subject

Now showing items 123-142 of 182

      Subject
      Oryza sativa; nitrogen; chlorophyll content; photosynthesis; yield components. [1]
      Pathogenic microorganisms, growth inhibition, ethanol extraction, secondary metabolites, leaves [1]
      pest resistance, seedling inoculation [1]
      Phaseolus vulgaris, soil moisture, Sorghum bicolor [1]
      photosynthetically active radiation;Saline conditions;stomatal conductance [1]
      Phytochemical Research [1]
      Phytochemical, antimicrobial, Cercospora zeae-maydis and Xanthomonas campestrispv. musacearum [1]
      Phytochemicals [1]
      Phytophthora colocasiae; Colocasiae esculenta; Severity; Accession; Region [1]
      plant density, leaf yield, fruit yield, seed yield, Solanum spp. [1]
      Plant diversity;Grazing;Rainfall;Moist savannah [1]
      Plant extract;Phytochemicals composition;Secondary metabolites [1]
      Plant tolerance, Stressors, Quantitative evaluation, Qualitative evaluation. [1]
      Potentials [1]
      quantitative trait loci (QTLs), rice, Striga hermonthica, resistance [1]
      rainfed rice, water deficit, growth, yield, chlorophyll fluorescence, protein, chlorophyll, NERICA rice [1]
      Ralstonia solanacearum (E.F. Smith) Yabuuchi, is the cause of bacterial wilt of tomato and one of the most severe pathogens of solanaceous crops with a very wide host range. There is little information on R. solanacearum strains in Maseno region in Kenya despite the fact that bacterial wilt of tomato is of economic importance. The objective of this study was to isolate the strains of R. solanacearum of tomato and characterize morphologically and biochemically Ralstonia solanacearum races and biovars from infected tomato plants in Maseno region. This study was conducted at Maseno University in the Department of Botany at Microbiology Laboratory. Ten diseased tomato plants from Maseno, Mariwa, Seme and Hollo were collected and cut into small sections (0.5–1cm). The plant sections were then plated onto Triphenyl tetrazolium chloride (TZC) media. Profiling of the pathogen was done morphologicaly using culture techniques; biochemical tests included Gram staining test, Potassium hydroxide test, Catalase oxidase test, Gas production test, Starch hydrolysis test and sugar utilization test. The races were then identified by pathogenicity test on wide host range. All the isolates had fluidal pinkish red centered colonies on TZC media, they were Gram negative, potassium hydroxide solubility positive, produced gas from glucose, ooze test positive and did not hydrolyse starch which is typical of R. solanacerum. R. solanacearum strains from infected tomato plants in Maseno region were race 3 biovar 1 and race 3 biovar 3. All isolates were pathogenic on tomato plants. [1]
      Ralstonia solanacearum; Triphenyl tetrazolium chloride; Tomato; Bacteria wilt; Profiling; Pathogen; Race; Biovar. [1]
      rds: 813C, Africa, biomass, CO2 chambers, ecosystem respiration, gross primary production, humid savanna, net ecosystem exchange, nitrogen, soil moisture, Th [1]
      Relative shoot length, Relative biomass, Riparian communities, Bio control, Maize germ, [1]